描述
Topoisomerase I (DNA-relaxing enzyme) catalyzes the removal of superhelical turns from covalently closed DNA by a transient breakage and rejoining of phosphodiester bonds. Topoisomerase I is active in the presence of EDTA.

Applications: Relaxing positively and negatively supercoiled DNA (1). Producing DNA topoisomers (2).

Source: Purified from calf thymus.

Performance and Quality Testing: Endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease, and phosphatase assays; conversion of super-coiled DNA to relaxed DNA.

Unit Definition: One unit catalyzes the conversion of 0.5 µg of superhelical Φ X174 RF DNA to a relaxed state in 30 min. at 37°C.

Unit Reaction Conditions: 50 mM Tris-HCl (pH 7.5), 50 mM KCl, 10 mM MgCl2 , 0.1 mM EDTA, 0.5 mM DTT, 30 µg/ml/BSA, 0.5 µg
Φ X174 RF DNA, and enzyme in 50 µl for 30 min. at 37°C.
规格
Enzyme: Topoisomerase
Product Size: 500 units
Shipping Condition: Wet Ice

产品货号： 38042024
来自LIFE

内容及储存

Store in freezer (-5 to -30°C).

产品说明文件：

https://tools.thermofisher.com/content/sfs/manuals/38042024.pdf

https://tools.thermofisher.com/content/sfs/manuals/38042016.pdf

产品详情链接：
http://www.genia-bio.com/productDetail.asp?mid=35&id=10472