产品编号 C3389 | CAS号 9000-81-1 | SIGMA
Acetylcholinesterase from Electrophorus electricus (electric eel)

Type VI-S, lyophilized powder, 200-1,000 units/mg protein
安全技术说明书
别名: AChE Acetylcholine acetylhydrolase Cholinesterase, Acetyl
EINECS 编号 232-559-3   Enzyme Commission (EC) Number 3.1.1.7  ( BRENDA | IUBMB )  MDL number MFCD00081268
POPULAR DOCUMENTS:  PRODUCT INFORMATION SHEET (PDF)  |  SPECIFICATION SHEET (PDF)
Analysis Note
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
Biochem/physiol Actions
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
包装
10000 units in poly bottle
500, 2000 units in glass bottle
Preparation Note
This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution.
Unit Definition
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
Physical form
含 Tris 缓冲盐的冻干粉
Application
The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity.1 The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride.2
General description
Molecular Weight: 280 kDa 
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Acetylcholinesterase (AChE), True, RBC or Erythrocyte Cholinesterase, E.C. 3.1.1.7, Analytical and Industrial Enzymes, Application Index,
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type   Type VI-S
form   lyophilized powder
composition   Protein, ≥60% biuret
solubility   20 mM Tris HCl buffer, pH 7.5: soluble1.0 mg/mL, clear(lit.)
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   −20°C

产品说明文件：http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/c3389pis.pdf

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