描述
pTEF1/Zeo is a 3.6 kb vector designed to allow you to create your own cloning and/or expression vectors that confer resistance to Zeocin™ in both E. coli and yeast strains. The vector contains the Sh ble Zeocin™ resistance gene driven by the Saccharomyces cerevisiae TEF1 promoter for selection in any yeast. In addition, the synthetic EM-7 bacterial promoter is present for selection in E. coli. Several unique restriction sites flank the ZeoCassette™ region to allow easy subcloning of the region or cloning of additional elements into pTEF1/Zeo. This vector is also useful for generating gene disruptions in yeast.
Promoter: TEF1
Vector Type: pTEF
Product Size: 20 µg
Cloning Method: Restriction Enzyme ⁄ MCS
Protein Tag or Fusion: Untagged
Selection Agent (Eukaryotic): Zeocin™
Antibiotic Resistance (Bacterial): Zeocin™ (ZeoR)
内容及储存
20 µg of pTEF1/Zeo is provided supercoiled and lyophilized with 125 mg of Zeocin™ . Store the vector and Zeocin™ at -20°C. All components are guaranteed stable for 6 months when properly stored. See polylinker sequences on the web.
产品链接:http://www.genia-bio.com/productDetail.asp?mid=35&id=32630