产品编号 T4648 |  CAS号 9002-04-4 |  Sigma  
凝血酶 来源于牛血浆

Thrombin from bovine plasma

lyophilized powder, 40-300 NIH units/mg protein (biuret) 
  
安全技术说明书

别名:  IIa 因子

 EINECS 编号 232-648-7 
 
 
 Enzyme Commission (EC) Number 3.4.21.5  ( BRENDA | IUBMB ) 
 
 
 MDL number MFCD00166053 
 
 

Popular Documents:  Datasheet (PDF)  |  Specification Sheet (PDF)

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

基于 Biggs 改性法的 NIH 分析过程使用 0.2mL 稀释血浆（由血浆与等量生理盐水混合制得）作为底物，以及 0.1mL 凝血酶样品（在含 1% 白蛋白缓冲液中保持稳定）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。

Biochem/physiol Actions

丝氨酸蛋白酶，选择性断开纤维蛋白原中的精氨酸-甘氨酸键，形成纤维蛋白以及纤维蛋白肽 A 和 B。

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Other Notes

查看更多关于 凝血酶的信息， 请访问www.sigma-aldrich.com/enzymeexplorer。

包装

1000, 10000, 100000 units in glass bottle

Preparation Note

Activated with bovine brain thromboplastin.

Physical form

Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess persistent hypocoagulability in patients with septic shock. 1 
Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Clinical Chemistry, Coagulation Proteins and Reagents,

更多...  
Quality Level    PREMIUM  
form    lyophilized powder  
composition    Protein, 40-60%  
Featured Industry    Diagnostic Assay Manufacturing  
shipped in    wet ice  
storage temp.    −20°C  
Gene Information    cow ... F2(280685)