产品编号 T4648 | CAS号 9002-04-4 | Sigma
凝血酶 来源于牛血浆
Thrombin from bovine plasma
lyophilized powder, 40-300 NIH units/mg protein (biuret)
安全技术说明书
别名: IIa 因子
EINECS 编号 232-648-7
Enzyme Commission (EC) Number 3.4.21.5 ( BRENDA | IUBMB )
MDL number MFCD00166053
Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
Frequently Asked Questions
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Analysis Note
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
基于 Biggs 改性法的 NIH 分析过程使用 0.2mL 稀释血浆(由血浆与等量生理盐水混合制得)作为底物,以及 0.1mL 凝血酶样品(在含 1% 白蛋白缓冲液中保持稳定)。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。
Biochem/physiol Actions
丝氨酸蛋白酶,选择性断开纤维蛋白原中的精氨酸-甘氨酸键,形成纤维蛋白以及纤维蛋白肽 A 和 B。
General description
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.
Other Notes
查看更多关于 凝血酶的信息, 请访问www.sigma-aldrich.com/enzymeexplorer。
包装
1000, 10000, 100000 units in glass bottle
Preparation Note
Activated with bovine brain thromboplastin.
Physical form
Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0
Application
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess persistent hypocoagulability in patients with septic shock. 1
Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Clinical Chemistry, Coagulation Proteins and Reagents,
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Quality Level PREMIUM
form lyophilized powder
composition Protein, 40-60%
Featured Industry Diagnostic Assay Manufacturing
shipped in wet ice
storage temp. −20°C
Gene Information cow ... F2(280685)