产品编号 A4262 |  CAS号 50-76-0 |  Sigma  
放线菌素 D

Actinomycin D

from Streptomyces sp., ≥95% (HPLC) 
安全技术说明书

别名:  放线菌素 C1 放线菌素 IV 更生霉素

经验分子式（希尔表示法） C62H86N12O16 
分子量 1255.42 
Beilstein Registry Number  605235 
EINECS 编号 200-063-6 
MDL number MFCD00005033 
PubChem Substance ID 24890842 
Popular Documents:  Datasheet (PDF)  |  Specification Sheet (PDF)

Application

Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells, and as an antibiotic in treatment of various malignant neoplasms, including Wilm′s tumour and the sarcomas. Recommended for us in cell culture applications at 1μg/mL.

Caution

This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actiomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.

包装

2, 5, 10, 25 mg in serum bottle

Preparation Note

Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.

Biochem/physiol Actions

Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information 
Related Categories A - K, Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics A-F,

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biological source    from Streptomyces sp.  
assay    ≥95% (HPLC)  
color    red, powder  
mp    251-253  
solubility    ethanol, DMSO: soluble  
storage temp.    2-8°C